![SOLVED: Using your Vmax =2.225 umol/min from the uninhibited data, calculate kcat (kcat = Vmax/[ET], where ET is the total enzyme concentration. The concentration of stock solution of enzyme used was 0.35mg/mL SOLVED: Using your Vmax =2.225 umol/min from the uninhibited data, calculate kcat (kcat = Vmax/[ET], where ET is the total enzyme concentration. The concentration of stock solution of enzyme used was 0.35mg/mL](https://cdn.numerade.com/ask_previews/37951912-45bb-4020-9a65-3c82938b63de_large.jpg)
SOLVED: Using your Vmax =2.225 umol/min from the uninhibited data, calculate kcat (kcat = Vmax/[ET], where ET is the total enzyme concentration. The concentration of stock solution of enzyme used was 0.35mg/mL
![How to calculate kcat from Lineweaver-Burk plot following RFU? - Biochemistry and Molecular Biology - Science Forums How to calculate kcat from Lineweaver-Burk plot following RFU? - Biochemistry and Molecular Biology - Science Forums](https://www.scienceforums.net/uploads/monthly_07_2015/post-112807-0-19721400-1437371820.png)
How to calculate kcat from Lineweaver-Burk plot following RFU? - Biochemistry and Molecular Biology - Science Forums
![Example of kinetic parameter. (A) The kcat measured for the hydrolysis... | Download Scientific Diagram Example of kinetic parameter. (A) The kcat measured for the hydrolysis... | Download Scientific Diagram](https://www.researchgate.net/publication/12877083/figure/fig1/AS:277438450946049@1443157889376/Example-of-kinetic-parameter-A-The-kcat-measured-for-the-hydrolysis-of.png)
Example of kinetic parameter. (A) The kcat measured for the hydrolysis... | Download Scientific Diagram
![Km, Kcat, and Kcat/Km values at three mesoporous materials, calculated... | Download Scientific Diagram Km, Kcat, and Kcat/Km values at three mesoporous materials, calculated... | Download Scientific Diagram](https://www.researchgate.net/publication/260034222/figure/tbl2/AS:669687365005340@1536677318861/Km-Kcat-and-Kcat-Km-values-at-three-mesoporous-materials-calculated-using-the.png)